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human holotransferrin  (R&D Systems)


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    R&D Systems human holotransferrin
    (A) . Experimental design for the assessment of iron in murine T-cells that were exposed to iron media conditions ranging from 0.001mg/mL – 0.625mg/mL <t>holotransferrin;</t> (B). Histograms showing the mass-frequency distributions of iron mass/cell for each holotransferrin condition from cells taken from each of three mice. Mass distributions are shown in attograms (ag) (10 -18 g); (C). Line plots showing the mean iron mass/cell per tenth percentile for each holotransferrin condition from each mouse; (D). Line plots presenting proportions of cell proliferation activity per iron condition (percentage of total cells dividing 0, 1 or 2+ times) with log 10 x-axes from each mouse; (E). Correlation plot presenting the geometric means from each distribution versus the iron condition for each mouse (with a log 10 x-axis). Logarithmic trend lines, associated equations and r 2 values are displayed for each profile. Error bars show the 95 th confidence intervals; (F). Line plot presenting the mean total amount of iron consumed by the cells from the three mice for each iron condition (with a log 10 x-axis). Error bars show the range of total iron calculated per mouse. (G). Scatter plot presenting the inverse correlation between the magnitude of cell proliferation during the live culturing period against the skewness of the single cell metallomic distributions for iron.
    Human Holotransferrin, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 48 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human holotransferrin/product/R&D Systems
    Average 93 stars, based on 48 article reviews
    human holotransferrin - by Bioz Stars, 2026-03
    93/100 stars

    Images

    1) Product Images from "Rapid and precise quantification of lymphocyte iron content by single cell inductively coupled plasma mass spectrometry"

    Article Title: Rapid and precise quantification of lymphocyte iron content by single cell inductively coupled plasma mass spectrometry

    Journal: bioRxiv

    doi: 10.1101/2024.11.11.623006

    (A) . Experimental design for the assessment of iron in murine T-cells that were exposed to iron media conditions ranging from 0.001mg/mL – 0.625mg/mL holotransferrin; (B). Histograms showing the mass-frequency distributions of iron mass/cell for each holotransferrin condition from cells taken from each of three mice. Mass distributions are shown in attograms (ag) (10 -18 g); (C). Line plots showing the mean iron mass/cell per tenth percentile for each holotransferrin condition from each mouse; (D). Line plots presenting proportions of cell proliferation activity per iron condition (percentage of total cells dividing 0, 1 or 2+ times) with log 10 x-axes from each mouse; (E). Correlation plot presenting the geometric means from each distribution versus the iron condition for each mouse (with a log 10 x-axis). Logarithmic trend lines, associated equations and r 2 values are displayed for each profile. Error bars show the 95 th confidence intervals; (F). Line plot presenting the mean total amount of iron consumed by the cells from the three mice for each iron condition (with a log 10 x-axis). Error bars show the range of total iron calculated per mouse. (G). Scatter plot presenting the inverse correlation between the magnitude of cell proliferation during the live culturing period against the skewness of the single cell metallomic distributions for iron.
    Figure Legend Snippet: (A) . Experimental design for the assessment of iron in murine T-cells that were exposed to iron media conditions ranging from 0.001mg/mL – 0.625mg/mL holotransferrin; (B). Histograms showing the mass-frequency distributions of iron mass/cell for each holotransferrin condition from cells taken from each of three mice. Mass distributions are shown in attograms (ag) (10 -18 g); (C). Line plots showing the mean iron mass/cell per tenth percentile for each holotransferrin condition from each mouse; (D). Line plots presenting proportions of cell proliferation activity per iron condition (percentage of total cells dividing 0, 1 or 2+ times) with log 10 x-axes from each mouse; (E). Correlation plot presenting the geometric means from each distribution versus the iron condition for each mouse (with a log 10 x-axis). Logarithmic trend lines, associated equations and r 2 values are displayed for each profile. Error bars show the 95 th confidence intervals; (F). Line plot presenting the mean total amount of iron consumed by the cells from the three mice for each iron condition (with a log 10 x-axis). Error bars show the range of total iron calculated per mouse. (G). Scatter plot presenting the inverse correlation between the magnitude of cell proliferation during the live culturing period against the skewness of the single cell metallomic distributions for iron.

    Techniques Used: Activity Assay

    (A) . Mean fluorescence intensity (MFI) of CD71 (transferrin receptor) versus the geometric mean single cell iron content (ag) for each population distribution, from each holotransferrin condition; (B). Mean fluorescence intensity (MFI) of CD25 (alpha chain component of the IL-2 surface receptor on T-cells) versus the geometric mean single cell iron content (ag) for each population distribution, from each holotransferrin condition; (C). Mean fluorescence intensity (MFI) of CD71 versus the geometric mean single cell calcium content (ag) for each population distribution, from each holotransferrin condition; (D). Mean fluorescence intensity (MFI) of CD25 versus the geometric mean single cell calcium content (ag) for each population distribution, from each holotransferrin condition. Error bars present the 95 th confidence intervals for each metallomic distribution determined by SC-ICP-MS.
    Figure Legend Snippet: (A) . Mean fluorescence intensity (MFI) of CD71 (transferrin receptor) versus the geometric mean single cell iron content (ag) for each population distribution, from each holotransferrin condition; (B). Mean fluorescence intensity (MFI) of CD25 (alpha chain component of the IL-2 surface receptor on T-cells) versus the geometric mean single cell iron content (ag) for each population distribution, from each holotransferrin condition; (C). Mean fluorescence intensity (MFI) of CD71 versus the geometric mean single cell calcium content (ag) for each population distribution, from each holotransferrin condition; (D). Mean fluorescence intensity (MFI) of CD25 versus the geometric mean single cell calcium content (ag) for each population distribution, from each holotransferrin condition. Error bars present the 95 th confidence intervals for each metallomic distribution determined by SC-ICP-MS.

    Techniques Used: Fluorescence



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    Image Search Results


    (A) . Experimental design for the assessment of iron in murine T-cells that were exposed to iron media conditions ranging from 0.001mg/mL – 0.625mg/mL holotransferrin; (B). Histograms showing the mass-frequency distributions of iron mass/cell for each holotransferrin condition from cells taken from each of three mice. Mass distributions are shown in attograms (ag) (10 -18 g); (C). Line plots showing the mean iron mass/cell per tenth percentile for each holotransferrin condition from each mouse; (D). Line plots presenting proportions of cell proliferation activity per iron condition (percentage of total cells dividing 0, 1 or 2+ times) with log 10 x-axes from each mouse; (E). Correlation plot presenting the geometric means from each distribution versus the iron condition for each mouse (with a log 10 x-axis). Logarithmic trend lines, associated equations and r 2 values are displayed for each profile. Error bars show the 95 th confidence intervals; (F). Line plot presenting the mean total amount of iron consumed by the cells from the three mice for each iron condition (with a log 10 x-axis). Error bars show the range of total iron calculated per mouse. (G). Scatter plot presenting the inverse correlation between the magnitude of cell proliferation during the live culturing period against the skewness of the single cell metallomic distributions for iron.

    Journal: bioRxiv

    Article Title: Rapid and precise quantification of lymphocyte iron content by single cell inductively coupled plasma mass spectrometry

    doi: 10.1101/2024.11.11.623006

    Figure Lengend Snippet: (A) . Experimental design for the assessment of iron in murine T-cells that were exposed to iron media conditions ranging from 0.001mg/mL – 0.625mg/mL holotransferrin; (B). Histograms showing the mass-frequency distributions of iron mass/cell for each holotransferrin condition from cells taken from each of three mice. Mass distributions are shown in attograms (ag) (10 -18 g); (C). Line plots showing the mean iron mass/cell per tenth percentile for each holotransferrin condition from each mouse; (D). Line plots presenting proportions of cell proliferation activity per iron condition (percentage of total cells dividing 0, 1 or 2+ times) with log 10 x-axes from each mouse; (E). Correlation plot presenting the geometric means from each distribution versus the iron condition for each mouse (with a log 10 x-axis). Logarithmic trend lines, associated equations and r 2 values are displayed for each profile. Error bars show the 95 th confidence intervals; (F). Line plot presenting the mean total amount of iron consumed by the cells from the three mice for each iron condition (with a log 10 x-axis). Error bars show the range of total iron calculated per mouse. (G). Scatter plot presenting the inverse correlation between the magnitude of cell proliferation during the live culturing period against the skewness of the single cell metallomic distributions for iron.

    Article Snippet: Human holotransferrin (R&D systems, 2914-HT-001G) was added at concentrations of 0.001 mg/mL to 0.625 mg/mL.

    Techniques: Activity Assay

    (A) . Mean fluorescence intensity (MFI) of CD71 (transferrin receptor) versus the geometric mean single cell iron content (ag) for each population distribution, from each holotransferrin condition; (B). Mean fluorescence intensity (MFI) of CD25 (alpha chain component of the IL-2 surface receptor on T-cells) versus the geometric mean single cell iron content (ag) for each population distribution, from each holotransferrin condition; (C). Mean fluorescence intensity (MFI) of CD71 versus the geometric mean single cell calcium content (ag) for each population distribution, from each holotransferrin condition; (D). Mean fluorescence intensity (MFI) of CD25 versus the geometric mean single cell calcium content (ag) for each population distribution, from each holotransferrin condition. Error bars present the 95 th confidence intervals for each metallomic distribution determined by SC-ICP-MS.

    Journal: bioRxiv

    Article Title: Rapid and precise quantification of lymphocyte iron content by single cell inductively coupled plasma mass spectrometry

    doi: 10.1101/2024.11.11.623006

    Figure Lengend Snippet: (A) . Mean fluorescence intensity (MFI) of CD71 (transferrin receptor) versus the geometric mean single cell iron content (ag) for each population distribution, from each holotransferrin condition; (B). Mean fluorescence intensity (MFI) of CD25 (alpha chain component of the IL-2 surface receptor on T-cells) versus the geometric mean single cell iron content (ag) for each population distribution, from each holotransferrin condition; (C). Mean fluorescence intensity (MFI) of CD71 versus the geometric mean single cell calcium content (ag) for each population distribution, from each holotransferrin condition; (D). Mean fluorescence intensity (MFI) of CD25 versus the geometric mean single cell calcium content (ag) for each population distribution, from each holotransferrin condition. Error bars present the 95 th confidence intervals for each metallomic distribution determined by SC-ICP-MS.

    Article Snippet: Human holotransferrin (R&D systems, 2914-HT-001G) was added at concentrations of 0.001 mg/mL to 0.625 mg/mL.

    Techniques: Fluorescence